MLL1/WARD complex of human MLL1 (GenBank Accession No. NM_005933,) a.a. 3745-end with N-terminal His-tag and MW= 27 kDa, human WDR5 (GenBank Accession No. NM_017588), a.a. 22-end with N-terminal FLAG-tag and MW= 35 kDa, human Ash2L (GenBank Accession No. NM_001105214), a.a. 2-end with N-terminal His-tag and MW= 61 kDa, human RbBP5 (GenBank Accession No. NM_005057), a.a. 2-end with N-terminal His-tag and MW= 60 kDa, and human DPY30 (GenBank Accession No. NM_032574) a.a. 2-end with N-terminal His-tag and MW=12 kDa individually expressed in an E. coli expression system.