The TNFR2:TNF-alpha[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of TNFR2:TNF-alpha. The key to this kit is the high sensitivity of detection of biotin-labeled TNF-alpha by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, TNFR2 is coated on a 96-well plate. Next, biotinylated TNF-alpha is incubated with TNFR2 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.

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