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PARP15-FL Chemiluminescent Assay Kit

●Purchase●DescriptionThefulllengthPARP15(PARP15-FL)ChemiluminescentAssayKitisdesignedtomeasurePARP15activityforscreeningandprofilingapplications

  • 產(chǎn)品型號(hào):78596
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2023-11-12
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The full length PARP15 (PARP15-FL) Chemiluminescent Assay Kit is designed to measure PARP15 activity for screening and profiling applications. PARP15 is known to catalyze the NAD-dependent ADP-ribosylation of histones. This Assay Kit takes advantage of the sensitive detection of biotinylated NAD+. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP15-FL enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

Illustration 1: PARP15-FL Chemiluminescent Assay Kit schematic

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