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PARP6 Chemiluminescent Assay Kit

●Purchase●DescriptionThePARP6ChemiluminescentAssayKitisdesignedtomeasurePARP6activityforscreeningandprofilingapplications

  • 產(chǎn)品型號(hào):80556
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2023-11-12
  • 訪  問  量:193
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Description

The PARP6 Chemiluminescent Assay Kit is designed to measure PARP6 activity for screening and profiling applications. PARP6 is known to catalyze the NAD-dependent ADP-ribosylation of histones. The key to the PARP6 Chemiluminescent Assay Kit is the biotinylated NAD+. With this kit, only three simple steps are required for PARP6 reactions. First, histone proteins are coated on a 96-well plate. Next, a biotinylated NAD+ mix (termed PARP Substrate Mixture) is incubated with the PARP6 enzyme in an optimized assay buffer. Finally, the plate is treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.

PARP6 Chemiluminescent Assay Kit

Figure 1. PARP6 Chemiluminescent Assay Kit schematic

*NOTE: As of April 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.

This product has been cited 2 times.

Synonyms
poly (ADP-ribose) polymerase 6, poly(ADP-ribosyl)transferase 6, PARP6
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